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Project Completion Report of Ms. Lata Gawade, National
Institute of Oceanography, RC, Visakhapatnam, India
Ms.
Lata Gawade, Junior Technical Assistant in the National Institute of
Oceanography, Regional Centre, Visakhapatnam, India, was affiliated with the
Leibniz Centre for Tropical Marine Ecology (ZMT), Bremen, Germany from 1st
February to 30th April 2009 under the Joint NAM S&T Centre - ZMT Bremen
Fellowship scheme for the year 2009. She worked under the supervision of Dr.
Ruben Lara on the topic: 'What are the different molecular weight fractions
of DOC in the coastal region?' Using various research techniques, including
mass spectrometer, elemental analyzers, spectrophotometer and DOC analyzer,
she analyzed the marine water samples for the suspended particulate organic
matter (SPOM), particulate organic carbon (POC), particulate organic
nitrogen (PON), and δ13Carbon isotope to know the distribution and sources
of organic matter in the water column of the western Bay of Bengal. The spectrophotometric method was standardized for the measurement of chitin
content in the suspended matter of water column. Water samples for SPOM
analysis were collected at 10 different depths i.e. 0, 5, 10,
20,30,50,75,100,150 and 200 m using 10 liters niskin bottles. Salinity and
temperature for water column was measured by using CTD (seabird). Carbon and
Nitrogen contents were determined with a CN analyzer (N A 2100, CE
Instruments). δ13Carbon isotope fraction in the CO2 produced was measured
with a delta plus MAT 253 mass spectrometer coupled with EA Flash 1112
series connected via conflo 11(Thermofinnigan) and the results expressed as
per mil (‰) deviations from the δ13C/δ12C ratio of the international
standard for soil calibration for CHNS (USA). It was concluded from these
studies that C:N ratio and isotopic δ13C fraction indicated a huge terregenous input in the study area of Bay of Bengal, as the δ13Corg values
were between -24 to -28 ‰. δ13Corg values of the deep water column (1000m)
POM samples showed the high impact of sinking flux as the δ13Corg were
between -26 to -28 ‰ almost similar to the upper water column. |
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