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Project Completion Report of Dr. Thwe Thwe Win,
Myanmar
Dr. Thwe Thwe Win,
Assistant Lecturer at Mandalay Technological University of Myanmar was
affiliated with the International Centre for Chemical and Biological
Sciences (ICCBS), Karachi, Pakistan from 1st September 2009 to 28th February
2010 as a Post-Doctoral Fellow under the Joint NAM S&T Centre - ICCBS
Fellowship scheme for the year 2009. She worked on 'Molecular Mechanism of
Known and Unknown Chemotherapeutic Agents on Human Carcinoma Cell Lines'
under the supervision of Dr. Huma Rasheed, Assistant Professor in the the
Panjwani Center for Molecular Medicine and Drug Research (PCMD) of ICCBS.
She learned cell culture techniques and performed all procedures from cell
seeding to cryo-preservation under the required culture conditions. She
treated the cells with various concentrations of drugs and determined the
viability and proliferation of the cells using trypan blue exclusion assay.
The cytotoxicity study was performed by fluorimetric assay at different time
intervals using spectrofluorometer and IC50 values of the compounds were
determined. She also gained experience of isolating DNA with Trizol Reagent
for DNA fragmentation using agarose gel electrophoresis; isolated RNA from
the adherent cells for gene expression through quantitative Real Time PCR;
and learned primer designing using Primer-3 program and designed a number of
primers related to cancer pathways. She also learned protein expression by
Western Blotting (SDS PAGE) and antiplatelet activity. During her short stay
at ICCBS she attended the First Nationwide Patch Clamp Workshop hosted by
PCMD on December 21-23, 2009. Dr. Thwe Thwe Win gained useful knowledge and
skills in advanced technologies related to Molecular Oncology during her
affiliation with ICCBS Karachi. |
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